Diatome

Diatome

Diatome (pronounced die-uh-tohm) refers to a specialized tool used in electron microscopy. Specifically, it is a microtome designed for ultra-thin sectioning of samples embedded in resins, particularly for transmission electron microscopy (TEM). The primary function of a diatome is to produce sections of extremely fine and consistent thickness, often ranging from 50 to 100 nanometers. These ultra-thin sections are then mounted on grids and examined under the high magnification of an electron microscope.

The name "diatome" is derived from the use of diamond knives in the microtome. Diamond knives, known for their extreme sharpness and durability, are essential for cutting such thin sections without causing significant damage or compression to the sample. Other cutting edges can be used, but diamond knives provide optimal performance.

The precision of a diatome allows researchers to observe cellular structures, tissues, and materials at a sub-cellular and molecular level. This makes it an indispensable tool in various fields, including biology, materials science, and nanotechnology. Careful operation and maintenance of the diatome and its diamond knife are crucial for achieving high-quality ultra-thin sections and reliable electron microscopy results.