β-Agarase

β-Agarase (beta-agarase) is a hydrolase enzyme that catalyzes the hydrolysis of β-1,4-glycosidic linkages in agarose, a linear polysaccharide composed of alternating β-D-galactose and 3,6-anhydro-α-L-galactose residues. Agarose is a major component of agar, a complex polysaccharide mixture derived from certain marine red algae (Rhodophyta). β-Agarases break down agarose into smaller oligosaccharides, primarily neoagarooligosaccharides (NAOs), such as neoagarobiose, neoagarotetraose, and neoagarohexaose.

These enzymes are found in a variety of marine microorganisms, including bacteria and fungi, as well as some terrestrial bacteria. The production of β-agarase is essential for these organisms to utilize agar as a carbon and energy source.

β-Agarases are classified under the glycoside hydrolase family GH16, GH50, GH86, GH98 and GH118 according to the CAZy (Carbohydrate-Active Enzymes) database. They typically have a molecular weight ranging from 30 kDa to 120 kDa, depending on the source organism.

β-Agarases have various applications in biotechnology and research, including:

• Protoplast preparation: Used in conjunction with other cell wall degrading enzymes to release protoplasts from algal cells.
• Agarose gel degradation: Used to dissolve agarose gels after electrophoresis, simplifying DNA or protein recovery.
• Production of neoagarooligosaccharides: Used to produce specific NAOs, which have potential applications in pharmaceuticals, cosmetics, and food industries due to their reported biological activities, such as anti-inflammatory, antioxidant, and prebiotic effects.
• Structural analysis of agarose and agaran: Used as a tool for determining the structure and composition of agarose and related polysaccharides (agaran).

The activity of β-agarase can be influenced by factors such as pH, temperature, and the presence of certain metal ions. Optimal activity varies depending on the specific enzyme and its source.