Superose

Superose is a size exclusion chromatography resin widely used in biochemistry and molecular biology for separating molecules based on their size and shape. It is a cross-linked agarose-based matrix that allows smaller molecules to enter the pores of the resin, delaying their elution. Larger molecules, unable to enter the pores, pass through the column more quickly.

The Superose resin is available in various forms with different cross-linking densities and particle sizes, allowing for separation of molecules within different molecular weight ranges. Common grades include Superose 6 and Superose 12, with the number generally indicating the degree of cross-linking. Higher numbers correspond to lower cross-linking and therefore larger pore sizes, suitable for larger molecules.

Superose is commonly employed in protein purification, particularly in the final polishing steps where high resolution separation based on native molecular weight is desired. It is also used for the analysis of protein aggregates, protein complexes, and other biomolecules. The resin exhibits low non-specific binding, contributing to high recovery and purity of target molecules.

Factors affecting the separation performance of Superose chromatography include flow rate, buffer composition, and sample volume. The choice of Superose grade depends on the size range of the molecules being separated and the desired resolution. Maintenance of the column, including cleaning and storage under appropriate conditions, is essential for optimal performance and longevity.