Pol Bouin

Pol Bouin (1870 – 1962) was a French histologist and embryologist, best known for his contributions to the study of the endocrine system, particularly the interstitial cells of the testis (Leydig cells). He collaborated extensively with Paul Ancel, and together they published significant research on the role of these cells in male sexual characteristics.

Bouin is also known for developing Bouin's fixative, a widely used histological fixative solution consisting of picric acid, formaldehyde (formalin), and acetic acid. This fixative is valued for its excellent preservation of cellular detail, particularly for tissues intended for microscopic examination. It is frequently employed in the preparation of samples for immunohistochemistry and in situ hybridization. While providing good morphological preservation, it can cause shrinkage of the tissue and may not be suitable for all types of histological studies.

His research helped to establish the field of endocrine embryology and contributed significantly to the understanding of hormonal regulation during development.