Fructan beta-(2,1)-fructosidase

Fructan beta-(2,1)-fructosidase (EC 3.2.1.152) is an enzyme that catalyzes the hydrolysis of beta-2,1 linkages in fructans. Specifically, it breaks down fructans by cleaving the terminal beta-D-fructofuranose residues from the non-reducing end of molecules containing beta-2,1-linked fructose chains. This activity results in the release of fructose and the shortening of the fructan chain.

This enzyme is found in a variety of organisms, including plants, fungi, and bacteria. Its presence is often associated with the ability of these organisms to utilize fructans as a carbon source. In plants, fructan beta-(2,1)-fructosidase plays a role in fructan metabolism, which can be important for stress tolerance (e.g., cold or drought) and carbohydrate storage.

The enzyme exhibits specificity for beta-2,1 linkages, distinguishing it from other fructan-degrading enzymes that target different linkages, such as beta-2,6 linkages. The systematic name for this enzyme is beta-D-fructofuranoside fructanohydrolase.

The products of the reaction are usually fructose and a fructan molecule with one fewer fructose residue. The enzyme's activity is influenced by factors such as pH and temperature, with optimal conditions varying depending on the source of the enzyme.

In summary, fructan beta-(2,1)-fructosidase is an important enzyme involved in the degradation of beta-2,1-linked fructans, playing key roles in carbohydrate metabolism and stress response in various organisms.