2024 World Aquatics Swimming Championships (25 m) – Women's 4 × 100 metre medley relay

The Women's 4 × 100 metre medley relay at the 2024 World Aquatics Swimming Championships (25 m) is a swimming event that is part of the short course world championships. It involves teams of four female swimmers, each swimming a different stroke in the following order: backstroke, breaststroke, butterfly, and freestyle. The event is contested in a 25-metre pool.

The medley relay is a team event where the aggregate time of the four swimmers determines the team's final time and ranking. The event is typically held as part of the championships program and features preliminary heats followed by a final. Teams qualify for the final based on their performance in the heats. Medals are awarded to the top three teams in the final. Specific rules regarding relay takeovers and stroke regulations as defined by World Aquatics (formerly FINA) apply to the event. The specific schedule and entry lists can be found on the official World Aquatics website.