2016 FINA World Swimming Championships (25 m) – Women's 400 metre individual medley

The 2016 FINA World Swimming Championships (25 m) – Women's 400 metre individual medley was a swimming event held as part of the 2016 FINA World Swimming Championships (25 m). This competition featured female swimmers completing 400 metres, using all four swimming strokes in the following order: butterfly, backstroke, breaststroke, and freestyle. The event was held in a short course (25-metre) pool. Medals were awarded to the top three finishers. The competition determined the world champion for this particular event within the short course format.