Bitter's career was primarily dedicated to informal education, particularly in museum settings. She served as the first Curator of Education at the Carnegie Museum of Natural History in Pittsburgh, a position she held from 1957 to 1989. In this role, she developed innovative educational programs, exhibits, and workshops designed to engage children and families with science and natural history. Her approach emphasized hands-on learning and active participation, significantly influencing the field of museum education.
Beyond her work at the Carnegie Museum, Bitter was instrumental in the creation and development of the Children's Museum of Pittsburgh. She served on its planning committee and later as an educational consultant, helping to shape its philosophy and exhibits. Her influence extended nationally through her involvement with professional organizations such as the American Association of Museums (now the American Alliance of Museums) and the Association of Science-Technology Centers.
Daisy Lee Bitter's legacy lies in her dedicated efforts to make learning accessible, enjoyable, and meaningful for children, both through the medium of television and in the interactive environment of museums. She passed away in 2021 at the age of 93.